ARTIFICIAL INSEMINATION WITH CHILLED SEMEN IN DOGS

master's thesis

• Title (croatian): Umjetno osjemenjivanje rashlađenim sjemenom u pasa
• Author: Ajda Zabret
• Mentor: Martina Lojkić (mentor)
• Mentor: Branimira Špoljarić (mentor)
• Committee member: Juraj Grizelj (predsjednik povjerenstva)
• Committee member: Ivan Butković (član povjerenstva)
• Committee member: Martina Lojkić (član povjerenstva)
• Committee member: Nikica Prvanović Babić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2022-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Clinical Sciences

Abstract

Umjetno osjemenjivanje (UO) omogućuje poboljšanje genetskih osobina kod različitih
pasmina pasa. Korištenje svježeg ohlađenog sjemena postalo je popularno među uzgajivačima
radi brojnih razloga. Pogodno je za kratkotrajno skladištenje i transport sjemena na kratke
udaljenosti, unutar 24-48 sati. Smanjuje parenje u srodstvu i stres uzrokovan putovanjem
životinje na parenje, korištenjem sperme genetski vrijednih mužjaka iz svih krajeva svijeta.
Isto tako, sprječava širenje spolno prenosivih bolesti. Ohlađeno sjeme čuva se na temperaturi
od 4°C, gdje ovisno o vrsti razrjeđivača ostaje sačuvano kroz duže vremensko razdoblje.
Razrjeđivači štite sjeme od temperaturnog šoka, osiguravaju energiju te održavaju stabilan pH
i osmolarnost. Najčešće se koristi Tris-fruktoza-citratni razrjeđivač s dodatkom žumanjka koji
omogućuje preživljavanje spermija kroz 3 do 10 dana. UO s ohlađenim sjemenom može se
izvesti različitim tehnikama kao što su duboko vaginalno osjemenjivanje ili pak intrauterino
osjemenjivanje koje se može izvesti transcervikalnim (TCI) ili kirurškim polaganjem sjemena
laparotomski ili laparoskopski. Iako se vaginalnim osjemenjivanjem postižu dobri rezultati,
TCI predstavlja metodu izbora radi veće stope skotnosti i veličine legla. Za uspješno
izvođenje UO potrebno je dobro poznavati anatomiju ženskog spolnog sustava i fiziologiju
spolnog ciklusa kuje, kako bi se odredilo optimalno vrijeme za osjemenjivanje i primijenila
najprikladnije tehnika osjemenjivanja, ovisno o pojedinačnom slučaju.

Abstract (english)

Artificial insemination is a useful method to improve genetic traits in different dog breeds.
The use of fresh, chilled semen has become very popular among dog breeders. Chilled semen
is especially suitable for short term storage and short distance transportation, i.e., 24-48 hours.
This method allows for reducing travel-related stress and inbreeding, by using genetically
superior dog semen all over the world. It is also a good way to prevent the spread of sexually
transmitted disease. By mixing semen with extender and cooling to 4°C, fertility remains
preserved for a long time. Semen extenders protect the spermatozoa from cold shock, provide
energy substrates and maintain constant pH and osmolarity. Mostly used extender is Trisfructose-citrate-egg yolk-based extender providing sperm longevity for 3-10 days. AI with
fresh chilled semen can be performed using different techniques, such as deep vaginal
insemination, or preferably by intrauterine insemination, which can be performed by using
non-surgical transcervical catheterization or by surgical semen deposition by laparotomy or
laparoscopy. Vaginal insemination provides adequate pregnancy rate, but intrauterine
insemination gives higher pregnancy rate and litter size. To perform AI successfully, anatomy
of female reproductive tract and physiology of estrus cycle of the bitch must be known so that
procedure could be performed at appropriate time and by using the most appropriate
insemination technique and place of semen deposition, according to the individual case.

• Keywords: pas; ohlađeno sjeme; razrjeđivači; optimalno vrijeme za parenje; tehnike osjemenjivanja
• Keywords (english): dogs; chilled semen; extenders; optimal breeding time; insemination technique
• Language: english
• URN:NBN: urn:nbn:hr:178:637837
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-10-10 12:56:20