Promjene biokemijskih pokazatelja u krvi tijekom treninga potražnih pasa uključenih u različite hranidbene režime: obrok na osnovi sirovog mesa i termički obrađena prešana hrana

master's thesis

• Title (english): Differences of Blood Biochemical Parameters in Search and Rescue Dogs during Fieldwork Included in Different Feeding Regimes: Raw Meat Based Diet and Processed Pressed Dog Food
• Author: Tomislav Bureš
• Mentor: Zoran Vrbanac (mentor)
• Mentor: Diana Brozić (mentor)
• Committee member: Damir Stanin (predsjednik povjerenstva)
• Committee member: Diana Brozić (član povjerenstva)
• Committee member: Zoran Vrbanac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Radiology, Ultrasound Diagnostic and Physical Therapy); Zagreb
• Defense date and country: 2019-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Cilj provedenog istraživanja bio je utvrditi promjene u biokemijskim pokazateljima u serumu pasa Hrvatske gorske službe spašavanja uključenih u različite hranidbene režime tijekom rada. Istraživanje je obuhvatilo 12 zdravih pasa (3.57±1.81) koji su podijeljeni u dvije skupine: prešana hrana (PRESS skupina) i obrok na osnovi sirovog mesa (BARF skupina). Hrane su se razlikovale po načinu prerade te po sastavu makronutrijenata: hrana na osnovi sirovog mesa u suhoj tvari sadržavala je bjelančevine, masti i ugljikohidrate u omjeru od: 45,2:32,2:5,5, a prešana hrana: 27,5:18,7:42,8. Uzorkovanje je vršeno jednom mjesečno u razdoblju od 4 mjeseca, dvaput na dan, prije i poslije radnog treninga Komisije za potrage i lavine HGSS-a.
Usporedbom rezultata prije i nakon treninga između skupina te unutar njih, utvrđeno je kako na razinu glukoze nije utjecao hranidbeni režim te je ostala u referentnom rasponu i nakon rada. Razina laktata unutar skupina se nije razlikovala nakon treninga, dok je u skupini BARF utvrđena niža razina bazalnog laktata u usporedbi sa PRESS. U BARF skupini utvrđen je prolazni porast u koncentraciji ureje i kreatinina, kao i pad anorganskog fosfata nakon treninga, dočim je u PRESS skupini utvrđena značajno viša bazalna razina kreatinina i razina anorganskog fosfata nakon treninga. Usporedbom rezultata unutar obje skupine utvrđen je značajan porast u enzimskoj aktivnosti CK, LDH i AST te značajan pad u koncentraciji ukupnog kolesterola i Mg nakon treninga. Nakon treninga, u BARF skupini utvrđen je porast u razini TAG u usporedbi sa PRESS skupinom.
Provedeno istraživanje ukazuje na različit metabolički odgovor pasa na obrok u čijem su sastavu različito zastupljeni makronutrijenti, također vidljive su promjene u biokemijskim pokazateljima kao odgovor na različit proces proizvodnje, poglavito kod razine anorganskog fosfata i kreatinina. Stabilna razina glukoze u krvi nakon dugotrajnog treninga ukazuje na opsežnu prilagodbu metabolizma dostupnom izvoru energije iz praćenih hranidbenih režima. Stoga se kod radnih potražnih pasa povećana sposobnost korištenja masti kao energetskog supstrata može smatrati prednošću. Biljezi mišićnog oštećenja ukazuju da aktivnost duljeg trajanja i različitog intenziteta rezultira opsežnim fiziološkim procesima i višim energetskim potrebama kod radnih potražnih pasa.

Abstract (english)

The aim of the study was to evaluate the changes in serum biochemistry values in Croatian Mountain Rescue Service dogs included in two different feeding regimes during stimulated fieldwork. There were 12 healthy dogs (age 3.57±1.81) included in the study, divided into two groups: pressed dog food (PRESS group) and raw meat-based diet (BARF group). The diets differed in macronutrients level: on a dry matter basis BARF diet consisted of protein, fat, and carbohydrate in the following proportions: 45.2:32.2:5.5 and kibble diet 27.5:18.7:42.8. During the period of 4 months, blood samples were obtained two times per month: baseline sample in the morning before feeding and the second one immediately after the fieldwork.
Results were compared between and within the two groups had shown that pre and post-exercise glucose level was not affected by the type of diet and remained within the reference range. Post-exercise serum levels of lactate within the groups did not differ significantly. Nevertheless, the basal level of lactate was significantly lower in BARF versus PRESS group. The transitory rise in urea and creatinine levels post exercise as well as lower level of inorganic phosphorus was noted in the BARF group, whereas higher basal levels of creatinine and higher post workout levels of inorganic phosphorus were measured in PRESS group. Results within both groups showed higher activity of CK, LDH and AST in both grups post-exercise, as well as lower levels of total cholesterol and Mg. Post-exercise level of TAG was higher in BARF group in comparison to PRESS group.
Conducted research reflects different metabolic response to feeding regimes with different macronutrient composition, as well as differences in biochemical parameters as response to processing procedure, especially visible through inorganic phosphate and creatinine level. Stabile glucose level after strenuous fieldwork would indicate excessive metabolic adaptations to the main source of energy provided through observed feeding regimes. An increased ability to utilize fat should be considered an advantage in a dog subjected to prolonged fieldwork in which fatty acid oxidation is used as main energy source. Muscle damage indicators suggest that long period of different intensity level workload in search and rescue dogs result in various physiological processes as well as overall increased muscle activity demands.

• Keywords: potražni psi; BARF; prešana hrana; biokemijski parametri; trening
• Keywords (english): canine; search and rescue; BARF; pressed dog food; biochemical profile; exercise
• Language: croatian
• URN:NBN: urn:nbn:hr:178:107239
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-09-16 06:58:15