UČINAK RADIOFREKVENCIJSKOG ELEKTROMAGNETSKOG ZRAČENJA FREKVENCIJE 2500 MHz NA GIBLJIVOST I MORFOLOGIJU IN VITRO OZRAČENOG SJEMENA NERASTA

master's thesis

• Title (english): EFFECT OF RADIO FREQUENCY ELECTROMAGNETIC RADIATION WITH A FREQUENCY OF 2500 MHz ON THE MOBILITY AND MORPHOLOGY OF IN VITRO IRRADIATED SEMEN OF BOAR
• Author: Kim Senaši
• Mentor: Ivona Žura Žaja (mentor)
• Mentor: Marinko Vilić (mentor)
• Committee member: Silvijo Vince (predsjednik povjerenstva)
• Committee member: Marinko Vilić (član povjerenstva)
• Committee member: Suzana Milinković-Tur (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2022-02-01, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences

Abstract

Radiofrekvencijsko elektromagnetsko zračenje (RF-EMZ) može utjecati na kvalitetu
sjemena u ljudi te plodnost. No, rezultati istraživanja RF-EMZ-a na reproduktivni sustav
različitih vrsta domaćih životinja, osobito nerasta su vrlo oskudna. Stoga cilj ovog istraživanja
bio je utvrditi učinak elektromagnetskog radiofrekvencijskog zračenja frekvencije 2500 MHza na in vitro ozračene uzorke sjemena rasplodnih nerasta, odnosno na gibljivost spermija te na
udio subpopulacija spermija primjenom analize glavnih komponenti i klaster analize prema
morfometrijskim pokazateljima glave i repa. Polučivanje ejakulata, odnosno prikupljanje
uzoraka izvodilo se metodom manualne fiksacije penisa od sveukupno 12 nerasta te su uzorci
sjemena svakog nerasta potom bili raspoređeni u Petrijeve zdjelice na pokusnu i kontrolnu
skupinu. Sjeme nerasta ozračeno je u gigahercnoj transverzalnioj elektromagnetskoj (GTEM)
komori na frekvenciji od 2500 MHz-a i jakosti električnog polja od 10 V/m u trajanju od dva
sata. Nakon ozračivanja, razmazi sjemena obojani su Spermac metodom u svrhu jasnije
vizualizacije morfoloških djelova spermija. Morfometrijska analiza provedena je uz pomoć
kompjuterskog programa SFORM. Razlika između pojedinačnih morfometrijskih pokazatelja
spermija kao i gibljivost spermija pokusne i kontrolne skupine nisu bili statistički značajni.
Ipak, uočena je smanjena gibljivost pokusne skupine (74,7 %) u odnosu na kontrolnu skupinu
spermija (85,7 %). Progresivna gibljivost spermija je bila statistički značajno smanjena
(p<0,001) u pokusnoj skupini spermija (35,0 %) u odnosu na kontrolnu skupinu (60,1 %).
Analizom glavne komponente otkrivene su 4 komponente (dužina i širina glave, pravilnost
glave te opseg srednjeg dijela), dok su klaster analizom otkrivene 2 subpopulacije spermija (S1
subpopulacija) koju karakteriziraju spermiji manje duljine i pravilnijeg oblika glave te manjeg
opsega spojnog dijela repa te (S2 subpopulacija) koju karakteriziraju spermiji veće duljine i
izduljenijeg oblika glave te većeg opsega spojnog dijela repa. Pokusna skupina imala je veći
udio S1 subpopulacije spermija (51,83 %) u usporedbi sa kontrolnom skupinom (48,7 %), kao
i manji udio S2 subpopulacije (47,54 %) naspram kontrolne skupine (52,47 %). Učinak
radiofrekvencijskog zračenja frekvencije od 2500 MHz-a može negativno utjecati na gibljivost
spermija kao i na uspješnost oplodnje.

Abstract (english)

Radiofrequency electromagnetic radiation (RF-EMZ) can affect semen quality and
fertility in humans. However, the results of RF-EMZ research on the reproductive system of
different species of domestic animals, especially boars, are very scarce. Therefore, the aim of
this study was to investigate the influence of electromagnetic radiofrequency radiation at 2500
MHz on in vitro exposed semen samples of breeding boars, ie on spermatozoa motility and on
the share of spermatozoa subpopulations using principal component and cluster analysis
according to morphometric parameters of head and tail. Ejaculation, ie collection of samples,
was performed by the method of manual fixation of the penis from a total of 12 boars, and
semen samples of each boar were then distributed in Petri dishes to the experimental and
control groups. Boar semens were irradiated in a gigahertz transverse electromagnetic (GTEM)
chamber at a frequency of 2500 MHz and an electric field strength of 10 V / m for two hours.
After irradiation, semen smears were stained with the Spermac method for the purpose of
clearer visualization of morphological parts of spermatozoa. Morphometric analysis was
performed with the computer program SFORM. The difference between individual
spermatozoa morphometric parameters as well as spermatozoa motility of the experimental and
control groups were not statistically significant. However, reduced motility of the experimental
group (74.7%) compared to the control group of spermatozoa (85.7%) was observed.
Progressive spermatozoa motility was statistically significantly reduced (p<0.001) in the
experimental group of spermatozoa (35.0%) compared to the control group (60.1%). The
analysis of the principal component revealed 4 components (head length and width, head
regularity and middle circumference), while cluster analysis revealed 2 spermatozoa
subpopulations (S1 subpopulation) characterized by spermatozoa of smaller length and more
regular head shape and smaller midpiece circumference and (S2 subpopulation) characterized
by spermatozoa of longer and more elongated head shape and higher circumference of the
spermatozoa midpiece. The experimental group had a higher share of S1 subpopulation of
spermatozoa (51.83%) compared to the control group (48.7%), as well as a lower share of S2
subpopulation (47.54%) compared to the control group (52.47%). The effect of radiofrequency
50
radiation at a frequency of 2500 MHz can negatively affect spermatozoa motility as well as the
success of fertilization.

• Keywords: nerasti; elektromagnetsko radiofrekvencijsko zračenje; morfometrijska analiza; subpopulacije spermija
• Keywords (english): boars; electromagnetic radiofrequency radiation; morphometric analysis; spermatozoa subpopulations
• Language: croatian
• URN:NBN: urn:nbn:hr:178:138673
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-03-01 09:37:24