Myxobolus sp. u akvariju držanog zlatnog karasa (Carassius auratus auratus)

master's thesis

• Title (english): Myxobolus sp. in Goldfish (Carassius auratus auratus) held in Aquarium
• Author: Ariana Mikulić
• Mentor: Emil Gjurčević (mentor)
• Committee member: Krešimir Matanović (predsjednik povjerenstva)
• Committee member: Andrea Gudan Kurilj (član povjerenstva)
• Committee member: Emil Gjurčević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2024-11-29, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

U ovom istraživanju utvrđene su morfološke karakteristike i morfometrijske vrijednosti
spora Myxobolus sp. izdvojenih iz zlatnih karasa držanih u akvariju, a porijeklom iz Italije. Nadalje,
opisane su i histopatološke promjene u aksijalnom mišićju invadirane ribe. Na osnovi
mikroskopske pretrage svježih preparata aksijalnog mišićja i položaja plazmodija unutar mišićnih
vlakana, vrsta je determinirana kao Myxobolus lentisuturalis. U području stvorene pseudociste
jasno se uočavaju različite patološke promjene. U središnjem dijelu zahvaćenog područja prisutna
je izražena likvefakcijska nekroza mišićnih vlakana dok na periferiji zahvaćenog područja mišićna
vlakna podliježu monofazičnoj segmentalnoj koagulacijskoj nekrozi. Kao reakcija na ozljedu i
oslobađanje velikog broja spora u okolno tkivo, promjene se javljaju i u potkožju i koži iznad
zahvaćenog područja što u konačnici dovodi do stvaranja ulkusa. Invazija nametnikom uzrokuje
značajne histopatološke promjene koje mogu dovesti do poremećaja u plivanju i predstavljaju
ulazna vrata za sekundarne bakterijske infekcije.

Abstract (english)

In this study, the morphological characteristics and morphometric values of the Myxobolus
sp. spores isolated from goldfish held in aquarium originating from Italy were determined.
Furthermore, histopathological changes in the axial musculature of infected fish were analysed.
Based on microscopic examination of squash preparations of the axial muscles and the position of
the plasmodia intracellularly within muscle cells, the species was identified as Myxobolus
lentisuturalis. In the area where the pseudocyst was formed, different pathological changes were
clearly visible. In the central part of the affected area, there was pronounced liquefactive necrosis
of muscle fibres, while in the peripheral part, muscle fibres undergo monophasic segmental
coagulation necrosis. As a reaction to injury and the release of a large number of spores into the
surrounding tissue, changes also occur in the subcutis and skin above the affected area, ultimately
leading to ulcer formation. The parasite infection causes significant histopathological changes that
could lead to abnormal swimming behaviour and serve as a portal of entry for secondary bacterial
infections.

• Keywords: zlatni karas; Carassius auratus auratus; Myxobolus lentisuturalis; histopatološke promjene
• Keywords (english): goldfish; Carassius auratus auratus; Myxobolus lentisuturalis; histopathological changes
• Language: croatian
• URN:NBN: urn:nbn:hr:178:681464
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-16 10:20:37