Određivanje mikrobiološke čistoće površina u dodiru s hranom metodom ATP- luminometrije

master's thesis

• Title (english): Determination of Microbiological Cleanliness of Food Contact Surfaces Using ATP-luminometry
• Author: Zvonimir Oštrić
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Tomislav Mikuš (predsjednik povjerenstva)
• Committee member: Vesna Dobranić (član povjerenstva)
• Committee member: Nevijo Zdolec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2025-02-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

Cilj je rada bio odrediti čistoću površina koje dolaze u dodir s hranom temeljem prisutnosti
ATP-a te usporediti s nalazom broja aerobnih mezofilnih bakterija. Uzorkovano je 10 površina
različitih materijala (drvo, premazano drvo, inoks, keramika, dlanovi) tijekom 4 uzorkovanja.
Na površini od 176 cm2
određivan je ukupni broj bakterija te enterobakterija standardnim ISO
metodama, kao i količina ATP-a (RLU; relative light units) pomoću luminometra. Površine su
uzorkovane u zatečenom stanju te nakon provedene dezinfekcije. Mikrobiološka čistoća
površina bila je sukladna propisanim mikrobiološkim kriterijima za objekte pod saniratnim
nadzorom i prije i nakon dezinfekcije. Promatrajući pojedinačne površine, razlike u količini
ATP-a prije i nakon dezinfekcije bile su statistički značajne (p<0,05) u slučaju površine
kuhinjskog stola, drvene stalaže (stalaža iz sirane) i radnog stola studenata, dok je ukupni broj
bakterija bio značajno manji (p<0,05) samo u slučaju kuhinjskog stola. Nakon provedene
dezinfekcije ni prosječna količina ATP-a ni broj bakterija nisu se ralikovali značajno među
ispitivanim površinama. Snažne korelacije između količine ATP-a i broja bakterija nije bilo ni
na nečistim (r=0,24) ni na dezinficiranim površinama (r=0,13).

Abstract (english)

The aim of the study was to determine the cleanliness of surfaces that come into contact with
food based on the presence of ATP and to compare it with the number of aerobic mesophilic
bacteria. 10 surfaces made of different materials (wood, coated wood, stainless steel, ceramics,
palms) were sampled in 4 sampling sessions. The total number of bacteria and enterobacteria
on an area of 176 cm2
was determined using ISO standard methods and the amount of ATP
(RLU; relative light units) using a luminometer. The surfaces were sampled in their original
state and after disinfection. The microbiological cleanliness of the surfaces met the prescribed
microbiological criteria for facilities under sanitary supervision both before and after
disinfection. Looking at the individual surfaces, the differences in the amount of ATP before
and after disinfection were statistically significant (p<0.05) in the case of the kitchen table, the
wooden shelves (shelves from the cheese factory) and the student working table, while the total
number of bacteria was significantly lower only in the case of the kitchen table (p<0.05). After
disinfection, neither the average amount of ATP nor the number of bacteria differed significantly
between the surfaces tested. There was no strong correlation between the amount of ATP and
the number of bacteria on unclean (r=0.24) or disinfected surfaces (r=0.13).

• Keywords: ATP; luminometrija; mikrobiološka čistoća
• Keywords (english): ATP; luminometry; microbiological cleanliness
• Language: croatian
• URN:NBN: urn:nbn:hr:178:337858
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-03-03 12:37:35