Antimikrobna aktivnost enterokoka izoliranih iz sirovog mlijeka

master's thesis

• Title (english): Antimicrobial Activity of Enterococci Isolated from Raw Milk
• Author: Dajana Crk
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Vesna Dobranić (predsjednik povjerenstva)
• Committee member: Lidija Kozačinski (član povjerenstva)
• Committee member: Nevijo Zdolec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Hygiene and Technology of Foodstuffs); Zagreb
• Defense date and country: 2017-05-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Enterokoki imaju važnu tehnološku i higijensku ulogu u proizvodnji hrane životinjskog podrijetla. U ovom istraživanju izoliran je soj Enterococcus faecalis 101 iz sirovog mlijeka te je ispitan njegov inhibicijski potencijal prema izolatima bakterije L. monocytogenes iz hrane. Agar spot i agar difuzijskim testom utvrđene su zone inhibicije rasta L. monocytogenes ATCC 7644 i 6 izolata L. monocytogenes iz hrane primjenom neutraliziranog nadtaloga kulture E. faecalis što može biti rezultat djelovanja enterocina. Smanjenje populacije L. monocytogenes ATCC 7644 za 4-5 log zabilježeno je i u tekućim hranilištima primjenom kulture E. faecalis 101 odnosno nadtaloga. Ovi povoljni preliminarni rezultati preduvjet su za daljnju karakterizaciju soja s obzirom na sintezu enterocina i moguću biozaštitnu ulogu u pokusnoj proizvodnji hrane.

Abstract (english)

Enterococci represents important foodborne bacteria from technological and hygienic point of view. In this study, Enterococcus faecalis 101 was isolated from raw milk and subjected to testing its antilisterial activity. Inhibition zones were observed toward L. monocytogenes ATCC 7644 and 6 food-originated strains of L. monocytogenes by agar spot and agar difusion methods by applying neutralized supernatant of E. faecalis. Reduction of L. monocytogenes ATCC 7644 by 4-5 log was found in liquid media inoculated with E. faecalis 101 culture and supernatant, respectively. These promising preliminary results are prerequisites for further characterization of the strain with regard to enterocin sinthesis and posible protective role in experimental food production.

• Keywords: Enterococcus faecalis; enterocin; Listeria monocytogenes; hrana
• Keywords (english): Enterococcus faecalis; enterocin; Listeria monocytogenes; food
• Language: croatian
• URN:NBN: urn:nbn:hr:178:878297
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 08:45:08