Određivanje osjetljivosti na antimikrobne lijekove izolata listeria monocytogenes iz hrane prijemjenom e-testa

master's thesis

• Title (english): Determination of Antimicrobial Susceptibility of Listeria Monocytogenes from Food by E-Test
• Author: Tomislav Rajković
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Vesna Dobranić (predsjednik povjerenstva)
• Committee member: Ivana Filipović (član povjerenstva)
• Committee member: Nevijo Zdolec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Hygiene and Technology of Foodstuffs); Zagreb
• Defense date and country: 2016-06-23, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

U radu je istražena osjetljivost na antimikrobne tvari bakterije Listeria
monocytogenes (n=26) izoliranih iz hrane i proizvodnih pogona primjenom E-testa.
Određene su MIC vrijednosti ampicilina, gentamicina, vankomicina, streptomicina,
linezolida, azitromicina, rimfapicina, oksacilina, trimetoprim/sulfametoksazola i
ciprofloksacina. Svi su izolati bili osjetljivi na klinički relevantne antimikrobne
lijekove gentamicin, streptomicin, azitromicin, rifampicin,
trimetoprim/sulfametoksazol i ciprofloksacin. Distribucija izolata L. monocytogenes
prema MIC vrijednostima bila je za većinu tvari u nižem rasponu u odnosu na
EUCAST podatke. Pet izolata (19,23 %) L. monocytogenes bilo je otporno na
najmanje jednu tvar, a svi su izolirani s radnih površina, sirovine i opreme u mesnoj
industriji. Dva su izolata bila otporna na vankomicin, po tri na linezolid i oksacilin, a
jedan na sve tri tvari. Jedan izolat je pokazao otpornost na vankomicin, linezolid i
ampicilin. Također, u otpornih izolata izmjerene su i visoke MIC vrijednosti za
azitromicin-rifampicin, ampicilin-oksacilin, odnosno streptomicin-linezolid. Rezultati
ukazuju na nisku razinu otpornosti L. monocytogenes iz hrane na klinički
relevantne antimikrobne lijekove. S druge strane nalaz višestruko otpornih L.
monocytogenes na radnim površinama i opremi u mesnoj industriji upućuje na rizik
prijenosa antimikrobne rezistencije kroz lanac proizvodnje hrane.

Abstract (english)

In this study the antimicrobial susceptibility of Listeria monocytogenes from food
and food processing facilities (n=26) was tested by E-test. Minimal inhibitory
concentrations of ampicillin, gentamicin, vancomycin, streptomycin, linezolid,
azitromycin, rifampicin, oxacillin, trimethoprim/sulphametoxazol and ciprofloxacin
were measured. All isolates were susceptible to clinically relevant antibiotics
gentamicin, streptomicin, azitromycin, rifampicin, trimethoprim/sulphametoxazol
and ciprofloxacin. Distribution of L. monocytogenes according to MIC levels ranged
for most agents in lower values compared to EUCAST data. Five isolates (19.23
%) of L. monocytogenes was resistant at least to one antimicrobial agents, and all
originated from raw material, food contact surfaces and equipment in meat
processing plant. Two isolates were resistant to vancomycin, three to linezolid and
oxacillin, and one to all three agents. One isolate showed the resistance to
vancomycin, linezolid and ampicillin. Also, in the case of resistant isolates, the
high-level MIC of azitromycin-rifampicin, ampicillin-oxacillin and streptomicinlinezolid
were recorded. Results show the low level of resistance toward clinically
relevant antimicrobials in L. monocytogenes from food. Contrary, the occurrence of
multi-resistant L. monocytogenes strains in meat-processing facility samples
indicates the risk of antimicrobial resistance transfer along the food producing
chain.

• Keywords: Listeria monocytogenes; hrana; antimikrobna rezistencija
• Keywords (english): Listeria monocytogenes; food; antimicrobial resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:178:326774
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-23 08:57:21