Prvi dokaz metilja paramphistomum leydeni i paramphistomum cervi u jelena običnog u Hrvatskoj

master's thesis

• Title (english): First Evidence of Paramphistomum Leydeni and Paramphistomum Cervi Flukes in Red Deer in Croatia
• Author: Marina Špehar
• Mentor: Magda Sindičić (mentor)
• Committee member: Franjo Martinković (predsjednik povjerenstva)
• Committee member: Dean Konjević (član povjerenstva)
• Committee member: Magda Sindičić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Game Biology, Pathology and Breeding); Zagreb
• Defense date and country: 2018-10-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine

Abstract

Paramfistomozu domaćih i divljih preživača uzrokuju dikseni metilji iz roda
Paramphistomum koji kao odrasli primjerci parazitiraju na sluznici predželudaca, te mogu
uzrokovati značajne ekonomske gubitke. Iako se smatra da su metilji iz roda Paramphistomum
prisutni u Hrvatskoj, te su prve epizootije paramfistomoze zabilježene 2006. i 2010. godine na
ovcama i govedima, ne postoje znanstveni podatci o pojavnosti kod divljih preživača. Stoga je
cilj našega rada bio utvrditi koja vrsta metilja parazitira u jelena običnog (Cervus elaphus) na
području Hrvatske. Istraživanje je provedeno na ukupno 24 uzorka metilja iz buraga
odstrijeljene košute. S obzirom da je razlikovanje metilja na temelju morfoloških osobitosti
nedostatno, koristili smo molekularnu analizu ITS2 regije ribosomske DNA. Utvrdili smo kako
22 analizirana uzorka pripadaju vrsti Paramphistomum leydeni, dok se jedan uzorak podudara
s Paramphistomum cervi. Naši rezultati ne isključuju prisutnost i drugih vrsta, no ovo je prva
molekularna identifikacija vrsta iz roda Paramphistomum u Hrvatskoj. Važno bi bilo provesti
sustavno istraživanje rasprostranjenosti i učestalosti paramfistomoze i kod divljih i domaćih
preživača u Hrvatskoj, zbog moguće sve važnije uloge ovog parazita.

Abstract (english)

Paramphistomosis of domestic and wild ruminants is caused by ruminal flukes from
genus Paramphistomum that parasites in fore stomachs and cause major economic losses. Even
though it is considered that flukes from Paramphistomum genus are present in Croatia and the
first epizootiology data was registered in 2006 and 2010 on sheep and cattle, there are no
scientific data about the appearance of Paramphistomum flukes in wild ruminants. The goal of
our work was to determine the exact species of flukes which parasite in red deer (Cervus
elaphus) in Croatia. Research was conducted on 24 samples which were found in the rumen of
a hunted doe. Considering the fact that differentiation of species based on morphological
characteristics is insufficient, we used the molecular analysis of ITS2 region of ribosomal DNA.
We have determined that 22 samples are concurring with genetic material of Paramphistomum
leydeni, while only one sample concurred with Paramphistomum cervi. Our results do not
exclude the presence of other species, but this is the first identification of species from
Paramphistomum genus in Croatia. It would be important to conduct a systematic research of
distribution and prevalence of paramphistomosis both in wild and domestic ruminants
considering the rising importance of this parasite.

• Keywords: jelen obični; Cervus elaphus; Paramphistomum leydeni; Paramphistomum cervi; ITS2
• Keywords (english): red deer; Cervus elaphus; Paramphistomum leydeni; Paramphistomum cervi; ITS2
• Language: croatian
• URN:NBN: urn:nbn:hr:178:036991
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-12-19 13:27:10