MODULACIJSKI UČINCI NATIVNOG PROPOLISA, PRIPRAVKA PLEMENITE PEČURKE I β-GLUKANA NA IMUNOSNE TE PROIZVODNE POKAZATELJE ODBIJENE PRASADI

doctoral thesis

• Title (english): MODULATORY EFFECTS OF NATIVE PROPOLIS, PREPARATION OF WHITE BUTTON MUSHROOM AND β-GLUCAN ON IMMUNE AND PRODUCTION PARAMETERS IN WEANED PIGS
• Author: Daniel Špoljarić
• Mentor: Maja Popović (mentor)
• Committee member: Gordan Mršić (predsjednik povjerenstva)
• Committee member: Frane Božić (član povjerenstva)
• Committee member: Damir Mihelić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; Zagreb
• Defense date and country: 2013, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Basic and Preclinical Veterinary Sciences
• Universal decimal classification (UDC): 612 - Physiology

Abstract

Modulacijski učinci nativnog propolisa, pripravka plemenite pečurke i β-glukana na imunosne
te proizvodne pokazatelje odbijene prasadi
Od 2006. godine, europske upute ograničavaju nekliničku uporabu antibiotskih poticatelja
rasta (APR) u proizvodnji životinja namijenjenih ljudskoj prehrani, pa tako i u svinja. Stalno
prisutni zdravstveni problemi u intenzivnom uzgoju svinja, do sada su bili rješavani
dodavanjem subterapijskih doza APR u hranu radi povećanja proizvodnosti i
preventive/kontrole crijevnih infekcija. Prilagođavanje na povlačenje APR iziskuje žurno
utvrđivanje relevantnih zdravstvenih kriterija i donošenje znanstveno utemeljenih
alternativnih strategija. Osim cijepljenja, te strategije uključuju uporabu modifikatora
imunosnog odgovora (MIO), prirodnih tvari s imunomodulacijskim i protubakterijskim
učincima. Stoga su ciljevi ove disertacije pronalaženje tehnički i gospodarski primjenjivih
alternativa APR u hrani, među odabranim MIO (propolis, pripravak plemenite pečurke i β-
glukan iz kvasca), temeljem njihovih učinka na imunosne (in vivo i in vitro), zdravstvene i
proizvodne pokazatelje u odbijene prasadi.

Abstract (english)

Modulatory effects of native propolis, preparation of white button mushroom and β-glucan on
immune and production parameters in weaned pigs
Aim: since 2006 European-wide directives are restricting non-clinical use of antibiotic growth
promoters (AGP) in food animal production, particularly in swine. The problem of increased
sanitary problems in intensive swine production have been overcome thus far by adding subtherapeutic
doses of AGP in-feed to enhance production efficiency and prevent/control enteric
infections. To accommodate the withdrawal of AGP it now becomes urgent to provide
relevant health criteria and scientifically founded recommendations for alternatives. Besides
vaccination, these strategies include use of immune response modifiers (IRMs), natural
substances with immunomodulatory and bactericidal properties. Thus, the objectives of this
dissertation are to find out technically and economically applicable alternatives to to in-feed
AGP among IRMs (propolis, preparation of white button and yeast's β-glucan), based on their
effects on immune (in vivo and in vitro), health and productivity parameters in weaned pigs.
Methodology: 90 weaned pigs (Swedish landrase x Yorkshire) 28 days old were divided in
three groups of 30 animal: group A served as a control group and was fed with standard food
for weaned pigs; group B was fed with standard food plus preparation of white button
mushroom, while group C recieved native propolis with standard food. The experiment lasted
28 days, animals were scaled once a week, and blood samples were collected on weekly base
(days 7, 14, 21 and 28), in order to establish productive, health and immunological status of
animals in research.
Results: group B had the best productive results (the best average weight on day 28). Both of
the tested preparations resulted in similar immunological effects (increased values of tested
immunological parameters in experimental groups compared to control group). Observed
health indicators were slightly better in both experimental groups compared to control group,
but the values were within physiological range for species included in research.
Conclusion: both experimental preparations showed equally good immunomodulatory effect,
but preparation of white button mushroom had better nutraceutical effect.

• Keywords: antibiotski promotori rasta; modifikatori imunosnog odgovora; odbijena prasad; plemenita pečurka; nativni propolis; ß-glukan.
• Keywords (english): antibiotic growth promotors; immune response modifiers; weaned pigs; white button mushroom; native propolis; β-glucan.
• Language: croatian
• URN:NBN: urn:nbn:hr:178:914305
• Promotion: 2013
• Study programme: Title: doctoral study of veterinary physiology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-04-16 12:37:08