Utjecaj ekstrakata aromatskog bilja Dalmacije na patogene bakterije iz hrane životinjskog podrijetla

master's thesis

• Title (english): The Effect of Dalmatian Aromatic Herbs Extracts on Pathogenic Bacteria from Food of Animal Origin
• Author: Lucija Klanac
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Vesna Dobranić (predsjednik povjerenstva)
• Committee member: Željka Cvrtila (član povjerenstva)
• Committee member: Nevijo Zdolec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Hygiene and Technology of Foodstuffs); Zagreb
• Defense date and country: 2021-02-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Veterinary Public Health and Food Safety

Abstract

U ovom diplomskom radu cilj je bio istražiti antimikrobni potencijal alkoholnih i uljnih
ekstrakata sezonskog ljekovitog i aromatičnog bilja s područja Dalmacije - bosiljka, kadulje,
lavande, ružmarina i smilja. Antimikrobni potencijal procijenjen je disk difuzijskim testom
prema patogenim bakterijama značajnima u sigurnosti hrane: Salmonella Infantis, ermA+
Staphylococcus haemolyticus, ermC+ Staphylococcus haemolyticus, Staphylococcus aureus,
Yersinia enterocolitica, Listeria innocua, Listeria welshimeri, Listeria ivanovii i Listeria
monocytogenes. Uljni ekstrakti nisu uzrokovali inhibiciju rasta, dok su alkoholni ekstrakti
pokazali učinak na devet od 14 testiranih sojeva. Najosjetljivijima su se pokazali stafilokoki i
Y. enterocolitica koji su bili inhibirani svim ekstraktima. Ekstrakt ružmarina je inhibirao najširi
spektar, odnosno sedam indikatorskih bakterija, a najmanje lavanda, njih troje. Ekstrakt smilja
je pokazao najsnažniju inhibicijsku aktivnost u vidu najširih zona inhibicije prema ermA+
Staphylococcus haemolyticus, ermC+ Staphylococcus haemolyticus, Staphylococcus aureus.
Nakon tri mjeseca pohrane, antimikrobnu aktivnost, iako oslabljenu, zadržali su ekstrakti
kadulje, ružmarina i smilja.

Abstract (english)

In this work, the aim was to investigate the antimicrobial potential of alcoholic and oil extracts
of seasonal medicinal and aromatic plants from Dalmatia - basil, sage, lavender, rosemary and
immortelle. Antimicrobial potential was evaluated by disk diffusion test against pathogenic
bacteria important for food safety: Salmonella Infantis, ermA+ Staphylococcus haemolyticus,
ermC+ Staphylococcus haemolyticus, Staphylococcus aureus, Yersinia enterocolitica, Listeria
innocua, Listeria welshimeri, Listeria ivanovii and Listeria monocytogenes. Oil extracts caused
no growth inhibition while alcoholic extracts showed effect on nine of the 14 strains tested.
Staphylococci and Y. enterocolitica, which were inhibited by all extracts, were found to be the
most sensitive. Rosemary extract inhibited the broadest spectrum, i.e. seven indicator bacteria,
and lavender the least, i.e. three of them. Immortelle extract showed the strongest inhibitory
activity in terms of broadest zones of inhibition toward ermA + Staphylococcus haemolyticus,
ermC + Staphylococcus haemolyticus and Staphylococcus aureus. After three months of
storage, the antimicrobial activity, although impaired, of sage, rosemary and immortelle
extracts was maintained.

• Keywords: aromatsko i ljekovito bilje; antimikrobna aktivnost; patogene bakterije iz hrane
• Keywords (english): aromatic herbs; antimicrobial activity; foodborne pathogens
• Language: croatian
• URN:NBN: urn:nbn:hr:178:260461
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-03-04 12:01:33