Karakterizacija bakteriocina enterokoka izoliranih iz hrane životinjskog podrijetla

master's thesis

• Title (english): Characterisation of Bacteriocins Produced by Enterococci Isolated from Food of Animal Origin
• Author: Nina Vukušić
• Mentor: Nevijo Zdolec (mentor)
• Committee member: Vesna Dobranić (predsjednik povjerenstva)
• Committee member: Željka Cvrtila (član povjerenstva)
• Committee member: Nevijo Zdolec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Veterinary Medicine; (Department of Hygiene and Technology of Foodstuffs); Zagreb
• Defense date and country: 2019-01-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Veterinary Medicine; Animal Production and Biotechnology

Abstract

Enterokoki imaju važnu ulogu u fermentaciji hrane te često pokazuju probiotička svojstva. U
ovom radu istražen je njihov antimikrobni učinak in vitro prema referentnim sojevima i
sojevima izoliranih iz hrane bakterija Listeria monocytogenes, Listeria ivanovii, Listeria
innocua, Yersinia enterocolitica i Salmonella Typhimurium. Korištena je agar difuzijska
metoda, a nadtalozi neutralizirani i tretirani proteazom. Enterococcus faecalis EF-101 je
pokazao snažno inhibicijsko djelovanje prema svim sojevima bakterije roda Listeria, kao i
soja Y. enterocolitica O:3. Karakterizacijom inhibitora utvrđeno je da se radi o tvari
proteinske strukture, moguće bakteriocina enterocina čija je aktivnost bila 1280 AU/ml.
Potrena su daljnja istraživanja radi potencijalne primjene enterocina kao prirodnog
konzervansa u očuvanju održivosti hrane i povećanja mikrobiološke sigurnosti.

Abstract (english)

Enterococci have an important role in food fermentation and often exhibit probiotic
properties. In this paper, their antimicrobial effect was investigated in vitro against reference
strains and strains isolated from the food - Listeria monocytogenes, Listeria ivanovii, Listeria
innocua, Yersinia enterocolitica and Salmonella Typhimurium. The agar diffusion method
was used, and the supernatants were neutralized and treated with protease. Enterococcus
faecalis EF-101 showed strong inhibitory activity against all strains of Listeria as well as Y.
enterocolitica O:3 strain. By the characterization of the inhibitor, proteinaceous nature was
found, a possible bacteriocin enterocin, whose activity was 1280 AU / ml. Further research
should be carried out to assess the potential use of enterocin as a natural preservative in food
conservation and protection.

• Keywords: enterocin; patogene bakterije; hrana životinjskog podrijetla
• Keywords (english): enterocin; bacterial pathogens; food of animal origin
• Language: croatian
• URN:NBN: urn:nbn:hr:178:011299
• Study programme: Title: Veterinary Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica veterinarske medicine (doktor/doktorica veterinarske medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-11-02 07:31:00